Endothelial Microparticle-Derived Reactive Oxygen Species: Role in Endothelial Signaling and Vascular Function

Figure 4

Effects of endothelial MPs on NO production (a, b) and ROS production (c) in cultured mouse aortic ECs. ECs were exposed to endothelial MPs (10⁵/mL, 4 hours) or endothelial MPs pretreated with the NADPH oxidase inhibitor apocynin (Apo MPs, 10⁵/mL, 4 hours). (a, b) NO production was measured in A23187 (10 μM) stimulated ECs using diaminofluorescein diacetate and fluorescence microscopy. MP treatment impaired A23187 stimulated NO production and this process was partially attenuated by NADPH oxidase inhibition in microparticles. versus unstimulated cells, versus stimulated control group, -12. (c) EC ROS production was assessed by DHE-HPLC. versus unstimulated cells, versus stimulated control group, -4.

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