Protective Effect of Tempol on Buthionine Sulfoximine-Induced Mitochondrial Impairment in Hippocampal Derived HT22 Cells

Figure 1

Analysis of BSO-induced oxidative stress, total glutathione levels, superoxide dismutase (SOD) activity, and cell morphology and viability in HT22 cells: representative graphs show (a) levels of 8-isoprostane (), (b) total antioxidant capacity (), (c) total glutathione concentration (), (d) SOD activity (), (e) representation of cell morphology (10x magnification) of control treated and BSO-treated HT22 cells at 0, 6, 14, and 18 h, (f) percentage of cell viability using hemocytometer (), and (g) cell viability using MTT assay following (A) 14 h and (B) 18 h of BSO treatment. Decrease in absorbance corresponds to lower cell viability (). , , and significantly different from control; and significantly different from BSO-treated group. Bars represent means ± SEM.

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