Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity

<div>Protective effect of Cr on mitochondrial <svg height="8.68572pt" id="M26" style="vertical-align:-0.0498209pt" version="1.1" viewbox="-0.0498162 -8.6359 17.0108 8.68572" width="17.0108pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 0V24L339 665L311 657L43 24V0H600ZM497 50H107L303 539L497 50Z" id="g113-133"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="641" vert-adv-y="641"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,7.413,0)"><path d="M699 659C666 654 622 640 595 610C579 591 569 572 562 530L552 472C530 342 504 307 403 302V529C403 604 411 617 483 622V650H238V622C310 617 318 604 318 529V302C232 308 194 347 172 471L161 531C153 573 145 592 131 609C103 643 59 654 25 659L15 632C62 611 63 583 73 524L85 452C97 383 116 347 155 316C199 282 255 269 318 267V122C318 46 310 33 233 28V0H489V28C412 33 403 46 403 122V267C470 269 518 283 559 315C599 346 618 378 632 450L646 519C658 580 665 608 709 632L699 659Z" id="g113-152"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="725" vert-adv-y="725"></glyph.data></g></svg> after oxidative challenge in differentiating C2C12 myoblasts. The mitochondrial <svg height="8.68572pt" id="M27" style="vertical-align:-0.0498209pt" version="1.1" viewbox="-0.0498162 -8.6359 17.0108 8.68572" width="17.0108pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 0V24L339 665L311 657L43 24V0H600ZM497 50H107L303 539L497 50Z" id="g113-133"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="641" vert-adv-y="641"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,7.413,0)"><path d="M699 659C666 654 622 640 595 610C579 591 569 572 562 530L552 472C530 342 504 307 403 302V529C403 604 411 617 483 622V650H238V622C310 617 318 604 318 529V302C232 308 194 347 172 471L161 531C153 573 145 592 131 609C103 643 59 654 25 659L15 632C62 611 63 583 73 524L85 452C97 383 116 347 155 316C199 282 255 269 318 267V122C318 46 310 33 233 28V0H489V28C412 33 403 46 403 122V267C470 269 518 283 559 315C599 346 618 378 632 450L646 519C658 580 665 608 709 632L699 659Z" id="g113-152"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="725" vert-adv-y="725"></glyph.data></g></svg> (normalized ratio TMRE/MTG) was evaluated immediately after oxidative stress (DifD1) as the mitochondrial fraction insensitive to oligomycin. 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity 

Figure 2 | Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity 