Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity

Figure 4

Effect of Cr on AMPK. (a) The cell lysates were separated by SDS-PAGE and analysed for pACC, pAMPK, and AMPK protein expression by Western blotting after the oxidative insult (DifD1); representative images were reported. Blots were also probed for tubulin protein as loading control. (b) The levels of phosphorylated AMPK versus total AMPK and (c) the levels of phosphorylated ACC versus tubulin were examined. Data are the mean ± SEM. ; compared to control ().

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