Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity

Figure 6

Effect of Cr on the mitochondrial proteomic pattern. Mitochondrial proteomic mapping from myogenic C2C12 cells at DifD3; (a) control cells; (b) H₂O₂-treated cells; and (c) Cr-supplemented myocytes treated with H₂O₂ as in (b). The arrows show differentially expressed proteins after H₂O₂ treatment. The major differences in H₂O₂-treated cells as compared to (a) and (c) were found in 1 and 2, mitochondrial aldehyde dehydrogenase-2; 3, protein disulfide isomerase; 4, dihydrolipoamide dehydrogenase; 5, Mn superoxide dismutase; 6, prohibitin; and 7, ATP synthase D chain. Images were representative of five independent experiments.

(a)

(b)

(c)