Modulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair Pathways

Figure 1

Morphological Rho activity and proliferation analyses of parental and clonal HeLa cell lines expressing RhoA-N19 or RhoA-V14 mutants after γ-radiation. (a) Morphology of parental and derived HeLa cell lines. (b) Immunoblotting of pull-down assays for active RhoA (RhoA-GTP) in different cell lines. (c) and (d) Growth curves (c) and clonogenic assays (d) in cell lines under positive or negative modulation of RhoA activity. (e) Cell cycle profiles by flow cytometry analysis (using PI staining) of HeLa cell lines after exposure to different doses of γ-radiation. Graphs display mean ± SD of at least three independent experiments. , , and between clones and parental HeLa cells in the same treatment conditions (by ANOVA).

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