Research Article
Alternatively Spliced Methionine Synthase in SH-SY5Y Neuroblastoma Cells: Cobalamin and GSH Dependence and Inhibitory Effects of Neurotoxic Metals and Thimerosal
Figure 2
Alternative splicing of MS mRNA in SH-SY5Y cells. (a) Domain-specific PCR with SH-SY5Y cell-derived RNA indicates absence of cap domain products. (b) PCR products with SH-SY5Y and human lymphoblast (LB) RNA. 288 bp product includes exons 19–21; 125 bp product includes only exon 21. (c) qRT-PCR analysis of exon pairs in RNA from SH-SY5Y cells. (d) qRT-PCR analysis of exon pairs in RNA from L18 glioblastoma cells. (e) Western blot analysis of MS in SH-SY5Y cells. and versus untreated group.
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