Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2

Figure 7

GSPB2-induced autophagy in the cultured granulosa cells. Granulosa cells were pretreated with GSPB2 (10 μmol/L) for 24 h and then with H₂O₂ (150 μM) for 6 h. (a) The relative expression levels of autophagy-related genes. (b) The induction of LC3-II expression by GSPB2 was examined using Western blotting. (c) Quantification of relative LC3-II protein levels by gradation analyses. Values are expressed as the mean ± SEM, , , and versus H₂O₂ alone group.

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