Bach1 Induces Endothelial Cell Apoptosis and Cell-Cycle Arrest through ROS Generation

Figure 4

Bach1 downregulation attenuates H₂O₂-induced cell apoptosis. HMVECs were transfected with Con siRNA or Bach1 siRNA for 72 hours and then treated with or without H₂O₂ (500 μM) for 12 hours; the levels of ROS (a) cell apoptosis (b) and the protein levels of cleaved caspase 3 and Bach1 (c) were evaluated (; , ).

(a)

(b)

(c)