Bach1 Induces Endothelial Cell Apoptosis and Cell-Cycle Arrest through ROS Generation

Figure 5

Bach1 disrupts cell-cycle progression and inhibits cell proliferation through ROS production. Cell proliferation was evaluated in (a) HMVECs that had been infected with Ad-GFP or Ad-Bach1, in (b) Ad-GFP- and Ad-Bach1-infected HMVECs treated with or without NAC (10 mM) for 48 hours, and in (c) HMVECs that had been transfected with Bach1siRNA or Con siRNA. Cells were cultured in 96-well plates, and Brdu incorporation assessments were performed via optical density measurements (450 nm wavelength) at the indicated time points (; , ). ((d) and (e)) HMVECs were infected with Ad-GFP or Ad-Bach1 and then incubated with or without NAC (10 mM) for 48 hours; then cells were harvested. The cell-cycle analysis was conducted using flow cytometry and quantified ().

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