Bach1 Induces Endothelial Cell Apoptosis and Cell-Cycle Arrest through ROS Generation

Figure 6

Bach1 regulates the expression of cell-cycle regulatory proteins through ROS production. (a) Western blot analysis of cell-cycle regulatory proteins (cyclin D1, cyclin E2, cyclin A2, cyclin B1, CDK2, CDK4, CDK6, p21, and p53) in Ad-GFP- and Ad-Bach1-infected HMVECs (; , ). (b) HMVECs were infected with Ad-GFP or Ad-Bach1 and then incubated with or without NAC (10 mM) for 48 hours; then cells were harvested. Cyclin D1, cyclin E2, and Bach1 protein levels were evaluated (; , ).