TGF-β and Hypoxia/Reoxygenation Promote Radioresistance of A549 Lung Cancer Cells through Activation of Nrf2 and EGFR

Figure 5

Combined treatment with TGF-β and hypoxia enhanced resistance to radiation in A549 cells. (a) Cells were pretreated with 1 ng/mL TGF-β and incubated with hypoxic medium for 2 h before IR (5 or 10 Gy), followed by reoxygenation. MTT assays were performed 48 h after IR. (b) Cells were transiently transfected with 10 nM of scramble-siRNA or Nrf2-siRNA for 24 h and were then subjected to combined treatment with TGF-β and hypoxia/reoxygenation. MTT assays were performed 48 h after IR.

(a)

(b)