Oxidative Medicine and Cellular Longevity

Research Article

The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress

Table 1

Intervertebral disc pathologies used for primary cell cultures.


Donor information			Experiments
Number	Gender, age	Level pathology, grade	Sublethal oxidative stress	Lethal oxidative stress

1	M, 35	L4/L5 Herniation IV	H₂O₂ sensitivity study, p21 time course Senescence validation 10 days: additive effect	H₂O₂ sensitivity study PI/A
2	F, 77	L4/L5 DDD III	p21 time course 10 days: additive effect	JC-1, MTT
3	F, 53	L4/L5 DDD IV	H₂O₂ sensitivity study, p21 time course Senescence validation 10 days: additive effect	H₂O₂ sensitivity study PI/A, MTT
4	F, 39	L5/S1 Herniation V	H₂O₂ sensitivity study Senescence validation 10 days: antioxidant	H₂O₂ sensitivity study PI/A, MTT
5	F, 31	L4/L5 Herniation uk	H₂O₂ sensitivity study Senescence validation	H₂O₂ sensitivity study JC-1, PI/A, MTT
6	F, 64	L4/L5 DDD III	H₂O₂ sensitivity study Senescence validation	H₂O₂ sensitivity study JC-1, PI/A, MTT
7	F, 53	L5/S1 Herniation IV	—	JC-1
8	M, 50	L4/5 DDD III	—	JC-1
9	F, 54	L5/S1 Herniation V	10 days: recovery, RT qPCR	—
10	M, 64	L5/S1 Herniation III	10 days: recovery, RT qPCR	—
11	M, 44	L5/S1 Her IV	10 days: antioxidant, RT qPCR	—
12	M, 56	L4/L5 Herniation III	10 days: recovery, RT qPCR	—
13	F, 51	C4/5 Herniation III	10 days: antioxidant	—
14	F, 42	L3/L4 Herniation III	10 days: antioxidant	—
15	F, 35	L5/S1 DDD, Herniation IV	10 days: recovery	—
16	M, 41	L4/L5 DDD III	10 days: additive effect	—
17	M, 40	L5/S2 DDD, Herniation V	10 days: antioxidant	—
18	M, 50	L4/L5 DDD, Herniation III	10 days: additive effect	—
19	F, 42	L3/L4 Herniation III	10 days: recovery	—
20	M, 43	L5/S1 DDD, uk	—	JC-1, bright field, MTT, MTT (I)
21	F, 51	L4/L5 DDD IV	—	JC-1, bright field, MTT, MTT (LY), MTT (I)
22	M, 47	L4/L5 DDD, Herniation III	—	JC-1, bright field, MTT
23	F, 44	L4/L5 DDD III	—	JC-1, bright field, MTT, MTT (LY), MTT (I)
24	F, 43	L5/S1 DDD, Herniation III	—	JC-1, bright field, MTT, MTT (LY), MTT (I)
25	M, 47	L4/5 DDD, Herniation III	—	Lethal: MTT (LY), MTT (I)
26	M, 39	L4/5 DDD, Herniation IV	—	Lethal: MTT (LY), MTT (I)
27	M, 21	L5/S1 Herniation III	—	Lethal: MTT (LY), MTT (I)
28	F, 43	L4/5 DDD, Herniation IV	—	Lethal: MTT (LY), MTT (I)
29	M, 33	L5/S1 Herniation III	—	Lethal: WB
30	F, 39	L5/S1 DDD, Herniation III	—	Lethal: MTT (LY), WB, MTT (I)
31	F, 39	L4/5 DDD, Herniation IV	—	Lethal: MTT (LY), WB, MTT (I)
32	uk	uk	—	Lethal: MTT (LY), WB
33	M, 55	L3/4 DDD III	—	Lethal: WB
34	F, 56	L3/4 Herniation IV	—	Lethal: MTT (LY), WB
35	M, uk	L4/5 Herniation uk	—	Lethal: MTT (LY)
36	uk	uk	—	Lethal: MTT (LY)

DDD: degenerative disc disease, PI/A: Propidium Iodide/Annexin staining, MTT: MTT assay, MTT (LY): MTT assay with LY, MTT (I): MTT assay with insulin, and uk: unknown.