HDAC6 Regulates the Chaperone-Mediated Autophagy to Prevent Oxidative Damage in Injured Neurons after Experimental Spinal Cord Injury

Figure 1

The expression of HDAC6 and LAMP-2a protein increased in the ASCI. 30 adult female C57BL/6J mice were divided into two groups: the T8 spinal cord hemitransected group (ASCI) and the sham operated group (Ctrl). (a) The expression of HDAC6 was analyzed by SP standing at 24 h after the operation ((A)-(B)). Brown represents HDAC6 positive expression in the cytoplasm, and its negative cell cytoplasm is light blue, while gray matter supports network negative staining; ((C)-(D)) TUNEL staining was used to observe neuronal apoptosis. (C) Field of vision is a small amount of TUNEL staining positive neurons and glial cells, which shows that only a small amount of neurons is undergoing apoptosis. Spinal cord tissue structure is basic intact, and there are few cavity formations. (D) Field of vision is more TUNEL staining positive neurons and glial cells (tan for its nucleus is positive), which shows that a large number of nerve cells are undergoing apoptosis. The structure of the spinal cord tissue is disorder, and there are more cavity formations. Scale bar: 20 μm. Semiquantitative analysis are consistent with the figure, and HDAC6 expression in ASCI was dramatically increased compared with the control group. At least 60 cells were included for analysis from five images per group. Values represent mean ± SEM, , versus corresponding control group. (b) By Western blot, in the ASCI group, the protein expression of HDAC6 and LAMP-2a was significantly higher than the control group. Values represent mean ± SEM, , versus corresponding control group. Each experiment repeated at least 3 times.

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