Research Article
Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells
Figure 3
ROS production in mES cells treated by 1 mM APO and 100 nM DPI computed from the automated time-lapse image acquisition of HE fluorescence for 120 minutes (a, b); selected single time point 60 minutes (c). HPLC determination of specific 2-OH-E(+) and nonspecific product E(+) of HE oxidation in the presence of 1 mM APO and 100 nM DPI (d, e). Data are presented as mean + SEM from four independent experiments. Statistical significance was determined by ANOVA post hoc Bonferroni’s Multiple Comparison test, . The groups marked by an asterisk are statistically significantly different from control.
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