Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

Figure 6

Effect of APO (1 mM) and DPI (10 nM) in absence and presence of antioxidant NAC (10 mM) on the Akt and Erk, phosphorylation and Nanog protein level in mES cells after 24 hours in complete medium. Total level of β-actin was used as a loading control. A typical representative western blot is shown.