Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

Figure 7

Effect of APO and DPI on Wnt pathway activity determined by TOPflash assay and GSK3 phosphorylation on S9. Effect of the Wnt3a conditioned media or exogenous nondegradable β-catenin on the transcriptional activation of a reporter gene (a). Effect of LY (10 μM), APO (1 mM), and DPI (10 nM) on the spontaneous (b), Wnt3a conditioned media induced (c), and exogenous nondegradable β-catenin-induced transcriptional activation (d). Data represent mean + SEM, from at least four independent experiments. Statistical significance was determined by ANOVA post hoc Bonferroni’s Multiple Comparison test (, , and ). Effect of LY, APO, and DPI on GSK3β (S9) phosphorylation (e). Total level of β-actin was used as a loading control. A typical representative western blot is shown.

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