CD38 Deficiency Protects the Heart from Ischemia/Reperfusion Injury through Activating SIRT1/FOXOs-Mediated Antioxidative Stress Pathway

Figure 3

Knockdown of CD38 reduces H/R-induced reactive oxygen species (ROS) generation in H9c2 cells. (a) Representative images of gating plot and peaking plot of intracellular ROS production. The intracellular ROS was stained with CM-H2DCFD and measured by flow cytometry assay. (b) Quantitative analysis of the ROS fluorescence intensity. The mean DCF of fluorescence intensity was summarized from different groups of the cells and the intracellular ROS production was markedly attenuated in CD38 knockdown cells compared with WT cells, regardless of stimulating with or without H/R. (c) The images of ROS detection. Fluorescence images of intracellular ROS in WT or CD38 knockdown H9c2 cells were captured using a fluorescent microscope with CM-H2DCFD staining when the cells were subjected to H/R, and a robust elevation of the intracellular ROS was shown in wild type cells, but not CD38 gene knockdown cells (magnification, ×100 scale bars, 200 μm; ).

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