Lower Superoxide Dismutase 2 (SOD2) Protein Content in Mononuclear Cells Is Associated with Better Survival in Patients with Hemodialysis Therapy

Figure 3

(a) Representative amplification curves of the quantitative real-time PCR for SOD2 and the housekeeping gene beta-actin in hemodialysis patients (HD, circles), patients with chronic kidney disease (CKD, squares), and control subjects (healthy; triangles). Curves with x-symbols indicate the no-template control in the PCR. (b) Melting analysis after amplification of SOD2 and the housekeeping gene beta-actin. The melting curve analysis confirmed the presence of one single peak in hemodialysis patients (HD, circles), patients with chronic kidney disease (CKD, squares), and healthy control subjects (healthy; triangles). Curves with x-symbols indicate the no-template control in the PCR and show a peak at lower temperature representing primer-dimers in the no-template reaction. (c) Example of size fractionation of PCR products on 1.0% agarose gels. M denotes the base pair DNA marker, lane 1 hemodialysis patient, lane 2 CKD patient, lane 3 healthy control, lane 4 negative control (no reverse transcriptase), and lane 5 negative control (no template). Left side shows PCR products for SOD2; right side shows PCR products for beta-actin.

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