Hepatocyte Growth Factor Reduces Free Cholesterol-Mediated Lipotoxicity in Primary Hepatocytes by Countering Oxidative Stress

Figure 4

HGF effect on antioxidants enzymes and GSH ratio. (a) Western blot and densitometric analysis of catalase and superoxide dismutase 1 (SOD1) under HGF treatment for different times. (b) Enzymatic activity of catalase and SOD, in Chow and HC cells treated with HGF for 24 h. (c) Ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG), determined by HPLC. Chow and HC cells were treated with HGF for different times. Each column represents the mean ± SEM of three independent experiments. (d) Western blot analysis of γ-gamma glutamyl cysteine synthetase (γ-GCS); GSH peroxidase (GPX) 3/4/5; mammal GSH-S-transferase (MGST); and glucose-6-phosphate dehydrogenase (G6PD). Images are representative of at least three independent experiments. Actin was used as housekeeping loading control. Each column represents mean ± SEM of at least four independent experiments. versus nontreated Chow cells; versus nontreated HC cells.

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