NLRP3 Inflammasome Activation in the Brain after Global Cerebral Ischemia and Regulation by 17<i>β</i>-Estradiol

<div>Proximity ligation assay demonstrating NLRP3-ASC complex formation after GCI and its suppression by E2. (a) Representative confocal images of proximity ligation assay (Duolink<i> in situ</i> co-IP) show that NLRP3-ASC complex is formed in hippocampal CA1 region of the young adult female rat at day 7 after GCI. This complex formation is robustly suppressed by E2 (magnification = 40x, scale bar = 50 <i>μ</i>m). (b) Quantification of fluorescence intensity using MATLAB software shows a statistically significant suppression of complex formation at day 7 after GCI (<svg height="15.3614pt" id="M11" style="vertical-align:-3.833899pt" version="1.1" viewbox="-0.0498162 -11.5275 49.4726 15.3614" width="49.4726pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z" id="g58-33"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="487" vert-adv-y="487"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,4.946,-1.651)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 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id="g113-54"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g></svg> sham versus GCI; <svg height="13.9365pt" id="M12" style="vertical-align:-3.834pt" version="1.1" viewbox="-0.0498162 -10.1025 50.6052 13.9365" width="50.6052pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: NLRP3 Inflammasome Activation in the Brain after Global Cerebral Ischemia and Regulation by 17<i>β</i>-Estradiol 

Oxidative Medicine and Cellular Longevity

NLRP3 Inflammasome Activation in the Brain after Global Cerebral Ischemia and Regulation by 17β-Estradiol

Figure 4