AP39, a Mitochondria-Targeted Hydrogen Sulfide Donor, Supports Cellular Bioenergetics and Protects against Alzheimer’s Disease by Preserving Mitochondrial Function in APP/PS1 Mice and Neurons

Figure 1

AP39 generates H₂S in WT neurons, primarily in the mitochondria. (a) The contribution of AP39 to H₂S production in neurons. Neurons from WT mice were treated with various concentrations of AP39 for 2 h, and H₂S production was detected by methylene blue assay. (b) Neurons were treated with different concentrations of AP39 for 2 h, and intracellular H₂S was detected using the fluorescent probe AzMC. DAPI was used to stain nuclei, and MitoTracker was used to stain mitochondria. The colocalization of H₂S with mitochondria was indicated by the overlapping of red (mitochondria) and green (H₂S) fluorescence in the merged image. Note the concentration-dependent increase in the H₂S signal in response to AP39 treatment. , compared with control treatment (no AP39).

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