Research Article
The Role of the Exo-Xis Region in Oxidative Stress-Mediated Induction of Shiga Toxin-Converting Prophages
Table 4
Duration of the lag phase of the phage lytic development after prophage induction with either hydrogen peroxide (1 mM) or UV irradiation (50 J/m2).
| | Strain |
The time range of the switch from lag to log phase | | H2O2 (1 mM) | UV (50 J/m2) |
| | MG1655 (λ) | 60–90 min | 60 min | | MG1655 (λΔexo-xis) | 90–120 min | 30–60 min | | MG1655 (λΔorfs) | 60–90 min | 30–60 min | | MG1655 (λΔorf60a) | 60–90 min | 30–60 min | | MG1655 (λΔorf63) | 60–90 min | 30–90 min | | MG1655 (λΔorf61) | 60–90 min | 30–60 min | | MG1655 (λΔorf73) | 30–60 min | 0–30 min | | MG1655 (λΔea22) | 60–90 min | 30–60 min | | MG1655 (λΔea8.5) | 60–90 min | 30–60 min | | MG1655 (Φ) | 60–90 min | 150–180 min | | MG1655 (ΦΔexo-xis) | a | 150–180 min | | MG1655 (ΦΔorfs) | 150–180 min | 150–180 min | | MG1655 (ΦΔorf60a) | 120–150 min | 90–120 min | | MG1655 (ΦΔorf63) | 120–150 min | 30–60 min | | MG1655 (ΦΔorf61) | 90–120 min | 30–60 min | | MG1655 (ΦΔorf73) | 90–120 min | 120–150 min | | MG1655 (ΦΔea22) | 120–150 min | 30–60 min |
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The value was not determined due to a very low efficiency of prophage induction under these conditions (as shown in Figure 2).
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