Parkin Protects against Oxygen-Glucose Deprivation/Reperfusion Insult by Promoting Drp1 Degradation

Figure 4

Inhibition of Drp1 by parkin overexpression confers protection against OGDR induced apoptosis and mitochondrial dysfunction. (a) Mouse N2a cells were untransfected or transfected with parkin-myc or vector, and western blot was performed to examine the expression of Drp1 and parkin proteins. Overexpression of parkin contributed to decreased expression of Drp1. (b, c) Mouse N2a cells untransfected or transfected with parkin-myc and Drp1-HA were subjected to 4 h OGD plus 12 h reperfusion. Enhanced expression of parkin was able to suppress OGDR induced apoptosis, which was abrogated by increased expression of Drp1. (d, e) Parkin overexpression improved cytochrome c oxidase and mitochondrial ATP synthase activities after OGDR injury, which was also attenuated by increased expression of Drp1. All data are representative of at least three independent experiments. Values are expressed as mean ± SEM. compared to control.

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