Efficacy of Mitochondrial Antioxidant Plastoquinonyl-decyl-triphenylphosphonium Bromide (SkQ1) in the Rat Model of Autoimmune Arthritis

Figure 6

SkQ1 promotes neutrophil apoptosis in absence and in presence of a survival signal provided by mitochondrial debris (MTD). Neutrophils were incubated for 2 h in the absence or presence of 2.5 mg/mL MTD, and then SkQ1 was added at the indicated concentrations. Apoptosis was measured 22 hours after addition of SkQ1. Seven independent experiments were done using blood of different donors. Percent of apoptotic cells in individual donors varied between 49 and 75%. -axis shows difference between percent of apoptotic cells in control and experimental wells. Not all tests were done for each serum, but each experimental group was reproduced 3–5 times with distinct blood samples; dots represent these distinct experiments. ANOVA with Dunn’s postcorrection, versus control; versus MTD-treated cells without SkQ1.