High Fat Diet-Induced Skeletal Muscle Wasting Is Decreased by Mesenchymal Stem Cells Administration: Implications on Oxidative Stress, Ubiquitin Proteasome Pathway Activation, and Myonuclear Apoptosis

Figure 6

Systemic mesenchymal stem cells (MSCs) administration prevents increased reactive oxygen species (ROS) levels in the tibialis anterior (TA) of mice fed with a high fat diet (HFD). C57BL/10J male mice were fed with a standard chow (control) or HFD for 38 weeks. At week 30, a subgroup of HFD mice received MSC injected through the tail-vein. At week 38, all mice were sacrificed. (a) Cryosections obtained from the TA were incubated with a DCF probe for ROS detection through fluorescence microscopy. Nuclei were labelled via Hoechst staining. (b) Quantification of ROS levels from experiments showed in (a). The values are expressed as the fold of induction of the DCF probe intensity. Values correspond to the mean ± SD (; versus control; versus HFD, two-way ANOVA).

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