Toll-Like Receptor 4 Reduces Oxidative Injury via Glutathione Activity in Sheep

Figure 1

Oxidative damage in sheep with transgenic TLR4 overexpression. (a) Southern blot analysis; Wt, wild type; 1–3 were transgenic sheep (Tg); (c) TLR4 plasmid. (b) Real-time PCR product analysis by agarose gel electrophoresis. TLR4 expression levels were different in transgenic animals; 1–9, transgenic sheep (Tg). (c) Exogenous TLR4 copies were measured by real-time PCR. (d) Relative quantitative analysis of TLR4 expression in cells from transgenic sheep after LPS stimulation. (e) MDA constant measurements. Data represent mean ± SE. ^a,b,cDifferent superscript letters indicate significantly different values between groups (). Wt, wild type; Tg-1, Tg-2, and Tg-3 were transgenic sheep that had 1, 2, or 3 copies of TLR4.

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