Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1/Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner

Figure 4

Brusatol reversed the protective effects conferred by Lipoxin A4. Representative photomicrographs (400x) showing H&E staining of intestine (a) and Chiu’s score (b) was carried out to evaluate the injury degree; quantitative analysis using ELISA method was taken to assay the concentration of oxidative marker 15-F2t-Isoprostane (c) and SOD activity (d) in intestine mucosa. Representative Western blots and quantitative analyses showing total Keap1 (e) and HO-1 (g) protein and nuclear Nrf2 (f) protein expressions in intestine mucosa. Each bar represents the mean ± SEM ( per group). , , one-way ANOVA with Tukey test.

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