Intermittent Hypoxia Affects the Spontaneous Differentiation In Vitro of Human Neutrophils into Long-Lived Giant Phagocytes

Table 1

Effects of hypoxia on giant phagocytes (G) area and markers.

Measures	N	IH	SH
Area of cells (µm^2); ()	1486 ± 129	1051 ± 101^†,††	1527 ± 158
Neutrophil elastase
relative % ()	100 ± 36.4	16.7 ± 3.8^†,††	58.1 ± 23.6
FI^#	35,888 ± 13,326	4,847 ± 1,003	19,383 ± 7,304
LysoTracker
relative % ()	100 ± 47.1	31.7 ± 9.1^†,††	116.7 ± 51.7
FI^#	17,501 ± 10,900	3,023 ± 1,748	21,633 ± 12,869
MitoTracker
relative % ()	100 ± 40	59.7 ± 25.6^†,††	98.2 ± 43.4
FI^#	11,582 ± 4,183	6,284 ± 2,759	11,459 ± 6,063
LC3B
relative % ()	100 ± 33.8	59.2 ± 20.8^†,††	81.8 ± 27.7
FI^#	34,246 ± 10,319	15,912 ± 6,962	27,078 ± 9,928
gp91-phox
relative % ()	100 ± 46.2	28.4 ± 15.4^†,††	65.1 ± 30.9
FI^#	19,886 ± 8,975	5,506 ± 2,459	13,072 ± 6,844
p22-phox
relative % ()	100 ± 41.5	45.0 ± 18.4^†,††	165.3 ± 54.9^†††
FI^#	18,384 ± 9,569	5,772 ± 2,962	34,938 ± 11,756

Freshly isolated PMN were exposed for 24 h to intermittent hypoxia (IH), sustained hypoxia (SH), or normoxia (N). Then, cells were cultured at normoxia for additional six days. Area of cells and fluorescence intensity (FI), defined as Raw integrated density (sum of pixel values)/Area of cells, was integrated with Image J 1.49k Software as indicated in Materials and Methods.
FI at normoxia was considered as 100% and the effects of IH and SH were calculated as relative % of normoxia for each of the indicated number of experiments.
^#FI representative data of one independent experiment. In each experiment at least 10 cells were counted at each condition.
^†Significance IH versus N, .
^††Significance IH versus SH, .
^†††Significance SH versus N, .