Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status

Figure 1

(a) 48 hr incubation of IPEC-J2 cells with S1P at different concentrations at 50, 100, and 200 ng/mL. Values represent average absorbance values of produced MTS formazan in metabolically active cells ± SEMs. No significant differences were found between control and cell monolayers exposed to S1P at concentration up to 200 ng/mL. (b) 48 hr incubation of IPEC-J2 cells with suramin at different concentrations at 50, 100, and 200 μM. Values represent average absorbance values of produced MTS formazan in metabolically active cells ± SEMs. Significant differences were found between controls and cell monolayers treated with higher concentrations (100 μM, and 200 μM, ) of suramin.

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