Functionalized Fullerene Increases NF-κB Activity and Blocks Genotoxic Effect of Oxidative Stress in Serum-Starving Human Embryo Lung Diploid Fibroblasts

<div>F-828 promotes an increase in the activity of NF-κB. (a) (qRT-PCR): changes in the levels of mRNAs encoding NFκB1 in HELFs. (b) (Fluorescent microscopy): photo of the cells stained with anti-p65 (FITC) antibodies and DAPI, ×40. (c) (FCA): (1): the FL1-NF-κB (p65) versus SSC plots. Gate R encircles the fraction of HELFs with elevated values of FL1-NF-κB (p65). (2): dependence of the median values of FL1-NF-κB (p65) signals on the fullerene concentration. (d) (FCA): (1): the FL1-NF-κB (pSer529) versus SSC plots. Gate R encircles the fraction of HELFs with elevated values of FL1-NF-κB (pSer529). (2): median values of the FL1-NF-κB (pSer529) signals as a function of the fullerene concentration.</div>

Oxidative Medicine and Cellular Longevity

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Figure 10

Figure 10: Functionalized Fullerene Increases NF-κB Activity and Blocks Genotoxic Effect of Oxidative Stress in Serum-Starving Human Embryo Lung Diploid Fibroblasts

Figure 10 | Functionalized Fullerene Increases NF-κB Activity and Blocks Genotoxic Effect of Oxidative Stress in Serum-Starving Human Embryo Lung Diploid Fibroblasts 