Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes

Figure 1

HaCaT cells were treated with or without different doses of Rapamycin ((a) 10, 20, and 40 nM), everolimus ((c) 50, 100, and 200 nM), Torin 1 ((e) 0.5, 1, and 2 μM), or pp242 ((g) 0.5, 1, and 2 μM) for 12 hours. Then, the HaCaT cells were treated with Rapamycin ((b) 20 nM), everolimus ((d) 100 nM), Torin 1 ((f) 1 μM), or pp242 ((h) 1 μM) for 4, 12, or 24 hours. Western blotting analysis was performed using primary antibodies against MTOR and phospho-Ser2481 mTOR. GAPDH served as a loading control. (i) HEKs were treated with or without Rapamycin (20 nM), everolimus (100 nM), Torin 1 (1 μM), or pp242 (1 μM) for 12 hours. HaCaT cells were treated with Rapamycin (20 nM), everolimus (100 nM), Torin 1 (1 μM), or pp242 (1 μM) for BrdU incorporation assay (j) and cell migration assay (k). The data were presented as means ± SD from three independent experiments and the representative figures were shown. Rapa: Rapamycin; Ever: everolimus; NS: nonsense.

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(i) HEKs

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