REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit

Figure 6

Cellular location and expression of HBD in HeLa cells. (a) Cells were transiently transfected with HA-pSG5-HBD (2 μg) + FRT-REGγwt, HA-pSG5-HBD (2 μg) + FRT-REGγN151Y (1 μg), and HA-pSG5-HBD (2 μg) + HA-pSG5 vector (2 μg). Cells were fixed and immunostained with anti-REGγ (green color) and anti-HBD (red color). Cell nuclei were stained with DAPI staining (blue color). Scale bar: 50 μm. (b) Percentage of HBD expression in HeLa cells with HA-pSG5-HBD + FRT-REGγwt, HA-pSG5-HBD + FRT-REGγN151Y, and HA-pSG5-HBD + HA-pSG5 vector. Each data represents the means of three independent experiments. Bars are the standard errors, . Significance was determined by Student’s t-test. versus control.

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