REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit

Figure 7

REGγ promotes HBD degradation under oxidative stress. HeLa shN and HeLa shR cells were transfected with or without HA-pSG5-HBD plasmid. Cells were treated with a 5 mM concentration of PHZ for 0, 1, and 2 h. Oxidative stress via PHZ stimulation on HBD in the presence or absence of REGγ was measured by Western blotting against anti-HBD and anti-REGγ antibodies. β-Actin was used as internal control. (b) Quantification of HA-pSG5-HBD- and PHZ-treated Western blot results expressed as the means ± SEM. versus control.

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