4-Hydroxychalcone Induces Cell Death via Oxidative Stress in MYCN-Amplified Human Neuroblastoma Cells

Figure 1

4HC has potent cytotoxic effects on several MYCN-amplified and non-MYCN-amplified cell lines. Cell viability measured by MTT assay (a, c, e, g, i) and cell death measured by LDH assay (b, d, f, h. j) on (a, b) SK-N-BE (2), (c, d) IMR-32, (e, f) SH-SY5Y, (g, h) HEK293t cells, and (i, j) primary cultures of E14 rat midbrain, treated with the indicated concentrations 4HC for 24 h or 3DIV for E14 cells. All data are ; independent experiments for A-H, independent experiments for (i) and (j); , , and versus control; one-way ANOVA with Tukey’s test.

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