4-Hydroxychalcone Induces Cell Death via Oxidative Stress in MYCN-Amplified Human Neuroblastoma Cells

Figure 2

MYCN-amplified NB cells are more sensitive to the effects of 4HC than non-MYCN-amplified cell lines. Percentage change in (a) cell viability measured by MTT assay and (b) cell death measured by LDH assay on the four indicated cell lines after treatment with 25 μM 4HC for 24 h. All data are ; independent experiments. , , and compared to SH-SY5Y cells and , , and compared to HEK283t cells; one-way ANOVA with Fisher’s LSD test. Representative phase contrast micrographs of (c) SK-N-BE (2) cells and (d) IMR-32 cells treated with the indicated concentrations of 4HC for 24 h. . Somal and nuclear areas as percentages of controls in (e) SK-N-BE (2) cells treated with 25 μM 4HC for 6 h or (f) IMR-32 treated with 10 μM 4HC for 6 h. All data are ; independent experiments. and compared to controls (Cont) for each parameter; Students test for each parameter in each cell type.

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