4-Hydroxychalcone Induces Cell Death via Oxidative Stress in MYCN-Amplified Human Neuroblastoma Cells

Figure 4

4HC-induced cell death affects oxygen consumption rate in NB cells. Mitochondrial oxygen consumption rate (OCR) of (a) SK-N-BE(2) and (b) IMR-32 cells treated with 50 μM 4HC or vehicle (MeOH) for 4 h, at various stages: initially, “basal respiration” cellular OCR; then, OCR after treatment with oligomycin, a complex V inhibitor (“ATP production”), then OCR after treatment with FCCP, a protonophore (“maximal respiratory capacity”), and finally, OCR after treatment with AMA (“spare capacity”. OCR at each of the four individual stages in (c-f) SK-N-BE (2) and (g–j) IMR-32 cells from the same experiment, presented as a percentage of vehicle. All data are , independent experiments. , , and versus untreated; ANOVA with Fisher’s LSD test, unpaired test.

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