Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction

Figure 4

HMGB1-RAGE interaction is involved in ROS accumulation. Representative images (a) and quantification of fluorescence intensity (b) of DCFA staining after an incubation of 72 h. ROS stains are green while nuclei stain is blue. Scale . Relative expression of mRNA (c) and protein level (d, e) of HO-1. Changes of mRNA (f) and protein level (g, h) of RAGE. All data were normalized to β-actin. HMGB1-RAGE binding activity (i, j) was determined by performing coimmunoprecipitation assay after incubation for 3 days. . , ; .

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