LncRNA LEGLTBC Functions as a ceRNA to Antagonize the Effects of miR-34a on the Downregulation of SIRT1 in Glucolipotoxicity-Induced INS-1 Beta Cell Oxidative Stress and Apoptosis

Figure 4

LEGLTBC directly interacts with miR-34a. (a) Levels of LEGLTBC from nuclear and cytoplasmic fractions of INS-1 cells were measured by qRT-PCR, and results showed that LEGLTBC is mainly located in the cytoplasmic fractions. (b) Bioinformatics prediction indicated that the LEGLTBC sequence contains the putative binding site of miR-34a. (c) The luciferase activity was measured in INS-1 cells after having been cotransfected with LEGLTBC-WT or LEGLTBC-MUT and miR-34a or miR-NC for 48 h. (d) The RIP assay was performed to confirm whether LEGLTBC and miR-34a could directly bind to AGO2 in INS-1 cells. (e) The expression of miR-34a in HG/PA-treated INS-1 cells was assessed by qRT-PCR analysis. (f) Bivariate correlation analysis was performed to clarify the relationship between LEGLTBC and miR-34a expression level. (g) qRT-PCR was used to assay miR-34a expressions in INS-1 cells transfected with LEGLTBC, si-LEGLTBC, or respective controls. Results are presented as of independent experiments. Conditions significantly different ().

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