LncRNA LEGLTBC Functions as a ceRNA to Antagonize the Effects of miR-34a on the Downregulation of SIRT1 in Glucolipotoxicity-Induced INS-1 Beta Cell Oxidative Stress and Apoptosis

Figure 6

SIRT1 knockdown abolished the protective effects of LEGLTBC. (a) Western blot analysis showed that LEGLTBC silencing or overexpressing positively regulated SIRT1 expression. Knockdown of SIRT1 increased the cleaved caspase 3 expression in HG/PA-treated INS-1 cells (b), which were associated with an obvious increase of apoptotic cells (c) and ROS generation (d). INS-1 cells transfected with vector, LEGLTBC, LEGLTBC+si-NC, and LEGLTBC+si-SIRT1 were treated with HG/PA, followed by the detection of SIRT1 and cleaved caspase 3 expression (e, f, and g) and ROS production (h). Results are presented as of independent experiments. Conditions significantly different ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)