Research Article
Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway
Figure 1
Effect of ZER on cell viability and altered MMP-1 and collagen III expressions in UVA-irradiated HSF cells. (a) Chemical structure of zerumbone (ZER). (b) Cells were pretreated with ZER (0, 2, 4, or 8 μM for 24 h) followed by UVA irradiation (3 J/cm2 for 27 min). 24 h after UVA exposure, the percentage of cell viability was measured by MTT colorimetric assay as described. The formula used to calculate the percentage of viable cells was . (c, d) HSF cells were pretreated with ZER (0, 2, 4, or 8 μM for 24 h), and the expression of MMP-1 and collagen III proteins in the absence (c) or presence (d) of UVA radiation was measured using the western blot method. Results were presented as of three or more assays. , , compared to untreated control cells; #, ## compared to UVA-irradiated cells.
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