Exosomal miRNA Let-7 from Menstrual Blood-Derived Endometrial Stem Cells Alleviates Pulmonary Fibrosis through Regulating Mitochondrial DNA Damage

Figure 3

The shuttling of exosomal Let-7 between MenSCs and recipient cells. (a) Total RNA was first extracted from mice lung tissues and detection of Let-7 content in the four groups as measured by qRT-PCR (). (b) MLE-12 cells were exposed to TGF-β1, exosome, or TGF-β1 plus exosome for 48 h. Then, Let-7 content in MLE-12 cells as evaluated by qRT-PCR (). (c) MenSC-derived exosome was labeled with PKH26 and exposed to MLE-12 cells. Fluorescence intensity in MLE-12 cells coincubated with MenSC-derived exosomes labeled with PKH26 or not was performed by immunofluorescence. (d) Relative quantitative of the fluorescence content of the PKH26-labeled exosomes in MLE-12 cells (). (e) Fluorescence expression analysis of Cy3-labeled Let-7 mimic in MLE-12 cells treated with MenSC-derived exosomes (cells were firstly transfected with Cy3 and Cy3-labeled Let-7 mimic). (f) Quantitative statistics of the fluorescence content of Cy3-labeled Let-7 mimic in MLE-12 cells (). Data is shown as the . and .

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