Exosomal miRNA Let-7 from Menstrual Blood-Derived Endometrial Stem Cells Alleviates Pulmonary Fibrosis through Regulating Mitochondrial DNA Damage

Figure 5

The targeting regulatory effect of Let-7 on LOX1. (a) Total protein was extracted from the lung tissue of saline, BLM, BLM plus exosome, BLM plus exosome and negative control of Let-7, and BLM plus exosome and Let-7 inhibitor groups. Then, the expression of LOX1 protein was detected by western blotting. GAPDH was served as the internal control. (b) The transcription level of LOX1 in the mouse lung tissue of each group was determined by qRT-PCR (). (c) MLE-12 cells were exposed to TGF-β1, TGF-β1 plus NC mimic, TGF-β1 plus Let-7 mimic, TGF-β1 plus NC inhibitor, and TGF-β1 plus Let-7 inhibitor. Then, the transcription level of LOX1 in these cells was determined by qRT-PCR (). (d) Protein expression of LOX1 was detected by WB in the above cells. (e) Schematic diagram of the binding site of LOX1 3-UTR to Let-7-5p and the mutation of LOX1 3-UTR. (f) Luciferase activity of LOX1 was measured in cells transfected with Let-7 mimic and LOX1 3-UTR plasmids (). Data is shown as the . and .

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