Exosomal miRNA Let-7 from Menstrual Blood-Derived Endometrial Stem Cells Alleviates Pulmonary Fibrosis through Regulating Mitochondrial DNA Damage

Figure 6

The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis and fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid and control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-β1, TGF-β1 plus exosome, TGF-β1 plus exosome and vector, and TGF-β1 plus exosome and LOX1 overexpression plasmid (). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 and ACO2, and NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, and NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the , . .

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