Impaired Mitochondrial Fusion and Oxidative Phosphorylation Triggered by High Glucose Is Mediated by Tom22 in Endothelial Cells

Figure 2

Effect of high glucose on the expression of genes regulating mitochondrial dynamics and mitochondrial function. (a) Representative Western blot and quantification analysis of the level of proteins regulating mitochondrial dynamics in each group. The expression of Fis1 and Mff increased significantly upon treatment with high glucose, whereas the expression of Mfn1 decreased significantly. No significant change was seen in the level of Mfn2, Opa1, or Drp1 (). (b) Representative Western blot and quantification analysis of the level of proteins that are involved in OXPHOS; the loading control was β-tubulin (). (c) Total ATP level in HUVECs exposed to different concentrations of glucose for 48 hr (). (d) MMP was analyzed by staining with JC1 and was quantified based on fluorescence intensities. MMP: mitochondrial membrane potential (). (e) Apoptotic cells were detected at 48 h by Annexin V/PI staining and flow cytometric analysis () (, , and vs. control). Control: normal culture medium; HG15: D-glucose 15 mmol/l; and HG30: D-glucose 30 mmol/l. All data are presented as the .

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