Impaired Mitochondrial Fusion and Oxidative Phosphorylation Triggered by High Glucose Is Mediated by Tom22 in Endothelial Cells

Figure 5

Tom22 overexpression restored mitochondrial morphology. HUVECs were transduced with LV-Tom22 vectors that expressed Tom22. The LV-Con vector was used as a vehicle control. (a) The overexpression of Tom22 was confirmed by real-time PCR (). (b) A Western blot analysis was conducted to evaluate the overexpression efficiency of LV-Tom22 (). (c) Cells were stained with MitoTracker. Scale bar: 10 μm. (d) Quantification of the form factor and aspect ratio of the mitochondrial networks (). (e) Representative images of mitochondria visualized by transmission electron microscopy. When Tom22 overexpression, mitochondria morphology returned to longer which shortened by high glucose. Scale bar: 200 nm (, , and vs. LV-Con; NS = not significant) All data are presented as .

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