Impaired Mitochondrial Fusion and Oxidative Phosphorylation Triggered by High Glucose Is Mediated by Tom22 in Endothelial Cells

Figure 6

Tom22 overexpression attenuated the mitochondrial dysfunction and apoptosis induced by high glucose. HUVECs were transduced with LV-Tom22 vectors that expressed Tom22. The LV-Con vector was used as a vehicle control. (a) Western blot and quantification analysis of the level of proteins regulating mitochondrial dynamics. The expression of Mfn1 decreased significantly, and the expression of both Fis1 and Mff increased significantly. No significant change in the expression of Mfn2, Opa1, or Drp1 was seen (). (b) Representative Western blot and quantification analysis of the level of proteins that involve in OXPHOS (). (c) Total ATP level in HUVECs (). (d) The MMP was analyzed by JC1 staining and quantified based on fluorescence intensities (). (e) Apoptotic cells were detected by Annexin V/PI staining (). All data are presented as (, , and vs. LV-Con; and vs. LV-Con+HG30).

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