Impaired Mitochondrial Fusion and Oxidative Phosphorylation Triggered by High Glucose Is Mediated by Tom22 in Endothelial Cells

Figure 9

Overexpression of Mfn1 could protect against pathological mitochondrial dynamics and dysfunction of HUVECs induced by high glucose. HUVECs were transfected with LV-Mfn1 vectors that expressed Mfn1. The LV-Con vector was used as a vehicle control. (a) Western blot analysis was conducted to evaluate the overexpression efficiency of LV-Mfn1 (). (b) Representative confocal microscopy images of HUVECs stained with MitoTracker® Deep Red. Scale bar: 10 μm. (c) Quantification of the form factor and aspect ratio of the mitochondrial networks (). (d) Apoptotic cells were detected by Annexin V/PI staining (). (e) Total ATP level in HUVECs (). (f) The MMP was analyzed by JC1 staining and quantified based on fluorescence intensities (). All data are presented as (, , and vs. LV-Con; , , and vs. LV-Con+HG30).

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