Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Figure 3

Effect of salidroside (SAL) on the mitochondrial biogenesis-associated PGC1α-TFAM signaling pathway. 2BS cells at 50PD were treated by salidroside (SAL) at various dosages or resveratrol (RES) at 10 μM for 24 h and then were harvested for western blot analysis. (a) Representative images were acquired from three different experiments. (b) Quantitative analysis of the protein levels of PGC-1α, NRF1, and TFAM. Bars represent relative protein levels counted as (the value for 50PD control was set as 1.0), where and stand for the optical density of β-actin ladder and sample ladder, respectively. The optical density for each ladder was calculated by the ImageJ software. Data were obtained from three independent experiments. versus the 50PD control group.

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