Apocynin Dietary Supplementation Delays Mouse Ovarian Ageing

Figure 1

Ovarian structure and follicle number. (a) H&E-stained representative midovarian sections from young and aged mice. Note yellow-brown palely stained multinucleated cells, located in stroma of reproductively aged mice (white arrowheads). (b) Representative images of Sudan Black staining and autofluorescence in the ovaries of aged mice. Multinucleated cells stain intensely with Sudan Black and emit strong autofluorescence, indicating age-related deposition of oxidized proteins and lipids. Average quantification of autofluorescence area ( per group). (c) Follicle distribution at midovarian sections of young and reproductively aged mice ( per group), showing a significant decrease in primary and primordial follicle number of older animals. Y: young; AG: aged; AA: aged apocynin; PM: primordial follicles; P: primary follicles; S: secondary follicles; SA: secondary antral follicles; CL: corpus luteum. . Data are presented as . , compared with young female mice.

(a)

(b)

(c)